2′,3′CyclicGAMPELISAKit標準曲線和部分文獻欣賞

2′,3′-Cyclic GAMP ELISA Kit基本信息：

Cat：#K067-H5/K067-H1

Assay Type：Competitive ELISA

Sample Types：Cell Lysates, Tissue Extracts, Tissue Culture Media

Sensitivity：0.048 pmol/mL

Species：2',3'-Cyclic GAMP is identical across species

Assay Duration：2.5 Hours

Samples/Plate：39 in Duplicate

Readout：Colorimetric, 450 nm

艾美捷2′,3′-Cyclic GAMP ELISA Kit 標準曲線：

2′,3′-Cyclic GAMP ELISA Kit檢測原理:

The 2′,3′-Cyclic GAMP ELISA Kit quantitatively measures 2’,3’-cGAMP present in lysed cells and tissue, EDTA plasma, and tissue culture media samples. The 2′,3′-Cyclic GAMP ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert before performing this assay.

Use our provided 2’,3’-Cyclic GAMP (cGAMP) standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the 2’,3’-cGAMP-peroxidase conjugate and the 2’,3’-cGAMP polyclonal rabbit antibody. Then incubate the mixture for 2 hours shaking at room temperature. The immunological reaction occurs between the anti-2’,3’-cGAMP polyclonal antibody, the 2’,3’-cGAMP antigen in the sample or standard, and the 2’,3’-cGAMP-peroxidase conjugate. As the 2’,3’-cGAMP concentration in the sample increases, the bound 2’,3’-cGAMP-peroxidase conjugate decreases, causing a decrease in signal and vice versa.

After the 2-hour incubation, wash away the excess 2’,3’-cGAMP-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound 2’,3’-cGAMP-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the 2’,3’-cGAMP concentration in the samples.

2′,3′-Cyclic GAMP ELISA Kit部分出版物欣賞：

Proximity labeling reveals OTUD3 as a DNA-binding deubiquitinase of cGAS

April 25, 2023

SPECIES: HUMAN |SAMPLE: SUPERNATANT

Pharmacological boosting of cGAS activation sensitizes chemotherapy by enhancing antitumor immunity

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SPECIES: MOUSE |SAMPLE: SUPERNATANT

A G-quadruplex stabilizer, CX-5461 combined with two immune checkpoint inhibitors enhances in vivo therapeutic efficacy by increasing PD-L1 expression in colorectal cancer

November 25, 2022

SPECIES: HUMAN, MOUSE |SAMPLE: CELL CULTURE

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