MethPrimerDesignPrimersforMethylationPCRs

Welcome to MethPrimer 

• MethPrimer is a program for designing bisulfite-conversion-based Methylation PCR Primer. Currently, it can design primers for two types of methylation mapping PCRs: 1) Methylation-Specific PCR (MSP) and 2) Bisulfite-Sequencing PCR (BSP) or Bisulfite-Restriction PCR.

• Input Sequence: DNA sequences in any format. No editing is required before input.

• Output: MethPrimer returns results in both text and graphic view.

How to cite MethPrimer: Li LC, Dahiya R. . Bioinformatics. 2002 Nov;18(11):1427-31.

(moderated) Join the CpG method forum to ask questions and share your knowledge on methylation related  methods, such as bisulfite modification, primer design, MSP, bisulfite sequencing PCR. 

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Acknowledgement: MethPrimer is based on on the well-known primer design program (Steve Rozen, Helen J. Skaletsky (1998) Primer3. Code available at   Copyright (c) 1996,1997,1998 Whitehead Institute for Biomedical Research. All rights reserved. ).  

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MethPrimer was developed by Dr. at Dr. , Veterans Affairs Medical Center and University of California, San Francisco.