DetectionofMicroRNAHeterogeneityinSingleCellsUsinganAutomated
Introduction MicroRNA (miRNAs) are short (18–24 nucleotides), non-coding RNAs that regulate gene expression by both disrupting messenger RNA (mRNA) stability and inhibiting mRNA translation. The expression of miRNA species in cellular populations is thought to drive downstream gene expression and protein functionality. Our goal was to determine the variability in miRNA expre......閱讀全文
Comparative－assessment－of－glycosylation－of－recombinant－human－...（一）
Comparative assessment of glycosylation of recombinant human FSH and highly purified FSHHong Wang, Xi Chen, Xiaoxi Zhang, Wei Zhang, Yan Li, Hongrui Y
使用CO2恒溫搖床解決人胚腎－293－(HEK293)－細胞結團問題
人胚腎 293 (HEK293)? 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體? (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 HEK293 細胞
ImmunohistochemistyFluorescence－Protocol1
MaterialsCytokine-specific Primary Antibodiesunlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'&
可靠的CCCadvanced－FN1無異源耗材支持人間充質干細...（二）
Materials and MethodsShort-term cell growth evaluationLonza? Poietics? human mesenchymal stem cells (hMSC-BM, PT-2501, Lonza) derived from normal ad
Transfecting－Plasmid－DNA－into－NIH3T3－Cells－Using－Lipofectamine?－LTX－Reagent
實驗概要Lipofectamine? ?LTX Reagent is a proprietary, animal-origin free formulation for the ?transfection of DNA into eukaryotic cells with low cytotoxic
alamarBlue?－Cell－Viability－Assay－Protocol
實驗概要Cell health can be ?monitored by numerous methods. Plasma membrane integrity, DNA ?synthesis, DNA content, enzyme activity, presence of ATP, and c
Apoptosis－Induction
IntroductionWhen studying induction of apoptosis via a cell surface molecule, it is important to first ascertain surface expression of the molecule of
Subculturing－Adherent－Cells
實驗概要The following protocol describes a general procedure for subculturing adherent mammalian cells in culture.主要試劑1. Complete growth medium, pre-warme
MicroRNA－Expression－Profiling－by－Bead－Array－2
Materials and MethodsCell Culture, Interferon Treatment, and RNA PrecipitationMelanoma cells (ME-15) were cultured in RPMI 1640 with L-Glutamine suppl
流式細胞儀技術專輯
Flow Cytometry Analysis?(Springer Lab, Harvard University)?Flow cytometry employs instrumentation that scans single cells flowing past excitation sour
CO2恒溫搖床解決人胚腎－293－(HEK293)－細胞結團問題（一）
人胚腎 293 (HEK293)? 細胞在重組蛋白表達中是最常見的宿主細胞。 這類細胞能夠表達大量的膜蛋白，如 G 蛋白偶聯受體? (GPCR) ，是無法在最常見的生物制藥生產宿主，如：中國倉鼠卵巢 (CHO) 細胞中作表達。 HEK293 雖然是蛋白表達的極好宿主，然而 HEK293 細胞
哺乳動物RNAi技術－Mammalian－RNA－Interference
Mammalian RNA InterferenceThomas TuschlLaboratory for RNA Molecular BiologyThe Rockefeller University, New York?Excerpted from?RNAi: A Guide To Gene S
使用CCCadvanced?FN1無異源耗材培養人多能干細胞（二）
Materials and MethodsCell culture conditions and surface transitionCryopreserved hiPSCs (SC102A-1, SBI?, USA) were initially thawed and pre-cultivat
a－pipeline－for－the－identification－of－intact－Nglycopeptides（七）
Complementary ion information provided by HCD- and CID-MS/MS. Both HCD- and CID-MS/MScould be used to optimize the glycopeptide identification. Rece
流式細胞儀技術專輯
?最方便的實驗干貨查詢工具微信掃碼進入「丁香實驗」小程序編輯：?嗚咽分享到：??????Flow Cytometry Analysis?(Springer Lab, Harvard University)Flow cytometry employs instrumentation that scan
CO2恒溫搖床解決人胚腎－293－(HEK293)－細胞結團問題（二）
Lysate preparation and western blottingProtein lysates were created by harvesting the cells from con?uent T-?asks or from suspension cultures at h
ELISPOT－(Enzymelinked－ImmunoSPOT)－實驗方法步驟2
Cytokine ELISPOT ProtocolDescriptioneBioscience ELISPOT Ready-SET-Go! reagent sets contain the necessary reagents for performing enzyme linked immunos
Combined－Flow－Cytometric－Measurement－of－Two－CellSurface－Antigens
INTRODUCTIONFlow cytometry is frequently used to assess nucleic acid content?in individual cells. Based on DNA content alone, however, cells?in the qu
E.－Immunohistochemi...
實驗概要We provide a ?guideline procedure and tips for staining of paraffin embedded sections, ?including antigen retrieval, chromogenic detection and flu
第八屆全國微全分析系統學術會議微納米生物分析專場
2013年5月16日-19日，由中國化學會主辦、廈門大學承辦、復旦大學、浙江大學協辦的為期四天的第八屆全國微全分析系統學術會議、第三屆全國微納尺度生物分離分析學術會議暨第五屆國際微化學與微系統學術會議在美麗的海濱城市廈門隆重召開。以下是
Methods－for－the－Measurement－of－a－Bacterial－Enzyme－Activity－in－Cell－Lysates4
The use of a micro-titre based colorimetric assay provided a third method for the study of ACTase activity, and the most useful for large scale measur
基于epMotion－5075t及KAPA文庫定量試劑盒的全自動NGS文庫...2
Results and DiscussionAutomated qPCR Setup Accuracy and PrecisionThe accuracy and precision of the qPCR setup was evalu ated based on standard curve
ImmunohistochemistyFluorescence－Protocol2
Suitable for use on single-cell suspensions from peripheral blood, lymphoid tissue or cultured cell-lines.Sample Preparation and FixationHarvest cells
GentleMACS/單細胞懸液制備文獻集錦
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? ? ? ? ? 種屬 處理組織 文章名稱 human ? ? ? ? ? ? ?
基于數字PCR的單分子DNA定量技術研究進展（四）
NGS 是一種識別和確認未知致病菌的前景廣闊的技術，然而其在生物防御和公共健康應用等方面的時效性，卻往往因為缺乏快速、有效、可靠的自動DNA樣品制備方法而受到限制。為了突破這種限制，Kim 等設計了一種基于流體分布元件的數字微流體(DMF) 平臺，使得多子系統模塊能夠進入自動NGS庫樣品
Protocol－for－intracytoplasmic－staining－of－cytokines－for－FACS－analysis
DescriptionProtocol for intracytoplasmic staining of cytokines for FACS analysis?Procedure1) Prepare spleen, lymph node or T cell clone cells as singl
Automated－Genomic－DNA－Extraction
實驗概要This section ?provides a general protocol for automated isolation of genomic DNA from ?10-20 μl blood samples in a 96-well format using the Charge
A－rapid,－quantitative－and－inexpensive－method－for－detecting－apoptosis2
Figure?1.Determination of apoptosis in transiently transfected murine [beta] tumor cells. (A) The number of apoptotic [beta]HC 13T tumor cells (% apop
JC1分析線粒體膜電位的方法
Analysis of Mitochondrial Membrane Potentialwith the Sensitive Fluorescent Probe JC-1?Andrea Cossarizza and Stefano Salvioli?Department of Biomedical
Coenzyme－A－Detection
實驗概要The experiment provided ?an ?easy, convenient assay to measure the CoA level in a variety of ?biological samples. In the assay, free CoA is specif