CadmiuminducesDNAsynthesisandproliferationinmacrophages
Exposure to divalent cadmium ions (Cd2+) is a known cancer risk factor, but the molecular mechanisms responsible for the inappropriate induction of cellular proliferation by cadmium are still being figured out. One cellular model used to study this process is macrophages grown in culture. In cultured macrophages, cadmium acts both at the cell surface and in the cytoplasm to induce proliferation. At the cell surface, ......閱讀全文
Cadmium－induces－DNA－synthesis－and－proliferation－in－macrophages
Exposure to divalent cadmium ions (Cd2+) is a known cancer risk factor, but the molecular mechanisms responsible for the inappropriate induction of ce
DNA合成（DNA－synthesis－）技術介紹
蛋白質概念提出：DNA多肽合成（DNA synthesis ）：按照預定核苷酸的順序，將脫氧核苷酸逐個進行人工連接合成DNA鏈的方法。目前多是采用固相合成法，即是在多聚體支持物上從3′端延伸核苷酸，可自動化操作。)目前，oligo DNA合成一般都采用固相亞磷酰胺三酯法合成DNA片段，此方法具有高效
cell－proliferation－assay
cell proliferation assaybefore start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until c
Invitro－Phagocytosis－Assay－of－Macrophages
IntroductionThe term phagocytosis itself describes its mean phage = engulfment; cytosis: cell process. In other words, phagocytosis is the cellular pr
Transfection－of－siRNA－oligos－to－peritoneal－macrophages
Use DeliverX Transfection from PanomicsModified protocol belowStep 1:? Make complexesNote:? Do not scale up more than 3xThaw siRNA and DeliverX reagen
Synthesis－of－plasmalogens
Plasmalogens are a class of glycerophospholipids that contain one vinyl-ester linked long chain alcohol group in place of one ester-linked fatty acid
Sucrose－Synthesis
The prodution of sucrose takes place in plants, to store energy produced during photosynthesis. The extraction of sucrose from plants like sugar cane
Starch－Synthesis
The primary carbohydrate used for energy storage in plants is starch, the equivalent of glycogen in animals. Both starch and glycogen are polymers of
Lactose－Synthesis
The synthesis of glycosidic bonds between monosaccharides requires energy input, energy that is provided by joining sugar monomers to a nucleotide suc
Inhibition－of－Cellular－Proliferation－by－Gleevec
The drug Gleevec (also known as imatinib mesylate or STI-571) was approved by the FDA in 2001 for the treatment of CML, chronic myeloid leukemia. Whil
MTT－Cell－Proliferation－Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondri
GentleMACS/單細胞懸液制備文獻集錦
? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? ? ? ? ? ? 種屬 處理組織 文章名稱 human ? ? ? ? ? ? ?
Inactivation－of－Gsk3－by－AKT－causes－accumulation－of－bcatenin
Lipopolysaccharide (LPS) from XX bacteria induces a wide range of inflammatory responses, including the response of alveolar macrophages to bacteria i
Labeled－Sphingomyelin－Synthesis
Synthesis reaction:1) Mix purified DMSM with cyclohexylamine and 14CH3I at a ratio of 1/1.1/1.3 in 5 ml of methanol.2) Allow reaction to proceed at ro
Synthesis－of－Cardiolipin－－phosphatidylinositol
Phosphoglycerides are lipids that contain glycerol with a phosphate ester on one end and two fatty acid ester side chains. Phosphoglycerides such as p
Library－cDNA－Synthesis
Library cDNA Synthesis1° cDNA SynthesisN.B:?During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should either
Protocol－for－dsRNA－Synthesis
實驗概要? ? ? ? We routinely produce dsRNA by in vitro transcription of a PCR generated DNA template containing the T7 promoter sequence on both ends
Guide－to－Cell－Proliferation－and－Apoptosis－Methods
Chapter 1: Cell Death - Apoptosis and Necrosis1.1Introduction21.1.1Terminology of cell death21.1.2Differences between necrosis and apoptosis31.1.3Apop
CellTrace?－CFSE－Cell－Proliferation－Kit
實驗概要The CellTrace? ?CFSE Cell Proliferation Kit provides a versatile and well-retained ?cell-tracing reagent in a convenient and easy-to-use form. The
SREBP－control－of－lipid－synthesis
Sterol-regulatory element binding proteins (SREBPs) play a key role in transcriptional regulation of cholesterol metabolism in response to cholesterol
NB2cell－proliferation－assay
before start:thaw cells from liquid nitrogen, grow in 75cc flask (T75) in Fischer's medium MM (maintenance medium) until content gets cloudy and s
Culture－of－Endometrial－Explants－to－Monitor－Synthesis
Reprinted from:Hansen, P.J and Betts, J.G. (1992) Culture of endometrial explants and peri-implantation conceptuses to monitor synthesis and secretion
Universal－RiboClone?－cDNA－Synthesis－System
Universal RiboClone??cDNA Synthesis SystemThe Universal RiboClone??cDNA Synthesis System contains the reagents required for the synthesis of double-st
De－novo－synthesis－of－pyrimidine－nucleotides
NON-Clickable Image?The pyrimidine ribonucleotides, those found in RNA, are uracil and cytosine, each containing the sugar ribose and a nitrogenous ba
Proliferation－Assay:－[3H]－Thymidine－incorporation
Proliferation Assay: [3H] Thymidine incorporationContributor: Suprya JayadevDate: September 13, 1994Labelling:1) Seed cells in 6 well plates ( in norm
Cytokines－and－Inflammatory－Response
Inflammation is a protective response to infection by the immune system that requires communication between different classes of immune cells to coord
MTAF1基因突變與藥物因子介紹
該基因編碼一種轉錄因子，誘導金屬硫蛋白和其他參與重金屬（如鎘、鋅、銅和銀）穩態的基因表達。該蛋白是一種核質穿梭蛋白，在重金屬暴露后積聚在細胞核中，并與含有金屬反應元件（MRE）的啟動子結合[由RefSeq提供，2008年7月]This gene encodes a transcription fac
MTF1基因編碼功能及結構描述
該基因編碼一種轉錄因子，誘導金屬硫蛋白和其他參與重金屬（如鎘、鋅、銅和銀）穩態的基因表達。該蛋白是一種核質穿梭蛋白，在重金屬暴露后積聚在細胞核中，并與含有金屬反應元件（MRE）的啟動子結合[由RefSeq提供，2008年7月]This gene encodes a transcription fac
Cells－and－Molecules－involved－in－local－acute－inflammatory－response
Inflammation has several distinct components, including the localized response at the site of tissue injury or infection. Tissue injury stimulates the
METS－affect－on－Macrophage－Differentiation
Terminal differentiation of cells is often accompanied by repression of cellular proliferation, suggesting that there is a mechanism by which these ce